Organization U.S. Environmental Protection Agency (EPA)
Reference Code EPA-SSP-0008-14-7-15-19
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The EPA Environmental Research and Business Support Program has an immediate opening for a full-time Microbiological Lab Research support at the EPA facility in Cincinnati, OH.
What the EPA project is about
The Environmental Futures Analysis Branch (EFAB) of the Systems Exposure Division (SED), National Exposure Research Laboratory (NERL), Office of Research and Development (ORD) at EPA develops decision frameworks and integrated tools to help communities make better decisions to sustain a healthy society and environment. A research team in SED is working collaboratively with EPA’s National Health and Environmental Effects Research Laboratory (NHEERL) to explore health effects, both beneficial and detrimental, associated with environmental exposures, such as living near green spaces or contaminated sites. In addition, this team assesses health risks associated with microbial contamination of public drinking water supplies and recreational water bodies.
What you will be doing
As part of the research team, you will support research under the Systems Exposure Division team, and provide technical laboratory support for research projects using various biomarkers of health and microbiome analysis in blood, saliva, and stool samples.
Experience with general laboratory safety practices;
Proficiency of general laboratory techniques, including aseptic and sterile technique;
Demonstrated experience in microbiological (or related) research;
Substantial practical experience beyond college course work and demonstrated proficiency in molecular biology research, including experience with DNA/RNA extractions, PCR, RT-PCR, and gel electrophoresis;
Proficiency with Microsoft Office applications (i.e., Excel, PowerPoint, Word, Outlook);
Experience with data processing, cleaning, quality controls and basic data analysis techniques, including fitting dose-response curves to serially diluted standards, producing plots and summary tables, checking for out-of-range observations, and manipulating datasets, at a level enabling the applicant to process data with a minimal amount of instruction and guidance from EPA employees;
Strong communication skills including written, oral, and presentation abilities; and
Demonstrated leadership skills and have experience working in a multi-disciplinary team.
Experience with using statistical analysis software, such as SAS or R.
How you will apply your technical skills supporting the lab
Contributing to preparation of Quality Assurance Project Plan, Standard Operating Procedures, analytical protocols;
Coordinating receipt and distribution of water, saliva, blood, and stool samples and associated documentation to the appropriate EPA laboratories;
Processing of samples received, including filtration, centrifugation, and extraction;
Preparation of microbiological media and supplements for analysis of culturable bacteria;
Analysis of biomarkers in human samples including antibody responses to specific pathogens, and biomarkers of immune, metabolic and neuroendocrine functions using various assay technologies including Enzyme-Linked Immunosorbent Assay (ELISA), kinetic assay, and LuminexTM multiplex suspension assays;
Nucleic acid extraction and molecular analyses using a variety of commercial kits;
Analysis of microbiome using molecular techniques such as, but not limited to, reverse transcription (RT) polymerase chain reaction (PCR), quantitative PCR, droplet digital PCR, and targeted gene sequencing;
Preparation of sequencing libraries for microbial (bacteria and viruses) analysis using a variety of molecular techniques and validated protocols, including DNase treatment, PCR, whole genome amplification, sample barcoding, product clean-up and normalization;
Maintaining careful and accurate records in designated laboratory notebooks; and
Maintaining inventory of supplies and preparing lists of supplies to be ordered, as needed.
Communications-related responsibilities shall include:
Participating as a member of a multi-disciplinary research team;
Interacting with EPA scientists and other members of the laboratory team; and
Documenting experimental and sample collection efforts;
May be asked to present work performed as a poster at a scientific conference and may be asked to assist with writing a manuscript or report describing the research.
Location: This job will be located at EPA’s Research facility in Cincinnati, OH.
Salary: Selected applicant will become a temporary employee of ORAU and will receive an hourly wage of $29.22 for hours worked.
Hours: Full time
Travel: Occasional overnight travel may be required.
Expected start date: This position is full-time and expected to begin in August 2019. The selected applicant will be a temporary employee of ORAU working as a contractor to EPA. The initial contract period is through May 14, 2020.
You will be supervised by a mentor who will provide day-to-day direction, as well as coach, advise and counsel the student, and review your work. The mentor for this position will be a federal EPA employee.
The work will be in a laboratory setting and may involve exposure to hazardous elements.
Be at least 18 years of age and
Have earned a Master’s degree in biology, microbiology, molecular biology, or a related field, or a related discipline from an accredited university within the last 24 months and
Be a citizen of the United States of America or a Legal Permanent Resident.
Citizenship: LPR or U.S. Citizen
Degree: Master's Degree or Doctoral Degree received within the last 24 month(s).
Life Health and Medical Sciences (47 )
I certify that I am at least 18 years of age; a recent graduate with a Master’s degree in biology, microbiology, molecular biology, or a related field from an accredited university or college within the last 24 months; a citizen or a Legal Permanent Resident of the United States of America; and not a current employee of EPA ORD or the spouse or child of an EPA ORD employee.
ORAU is an Equal Opportunity Employer (EOE AA M/F/Vet/Disability); visit the ORAU website for required employment notices.